1X6P
Structure 4; room temperature crystal structure of truncated pak pilin from Pseudomonas aeruginosa at 1.63A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-15 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 38.114, 38.114, 149.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 1.630 |
| R-factor | 0.14056 |
| Rwork | 0.140 |
| R-free | 0.15765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dzo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.336 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.530 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.049 | 0.190 |
| Number of reflections | 14500 | |
| <I/σ(I)> | 26.7 | 10.1 |
| Completeness [%] | 99.5 | 89.6 |
| Redundancy | 7.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 293 | 60% Ammonium Sulfate, 0.1M Hepes, pH 8.2, VAPOR DIFFUSION, temperature 293K |
