1X6O

Structural Analysis of Leishmania braziliensis eukaryotic initiation factor 5a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-12
Detector	ADSC QUANTUM 315
Spacegroup name	P 32 2 1
Unit cell lengths	64.991, 64.991, 88.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.1765
Rwork	0.174
R-free	0.21398
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.624
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	28757
<I/σ(I)>	12.2	2.7
Completeness [%]	99.0	99.9
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	PEG 400, Potassium thiocyanate, Sodium Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	4	277	PEG 400, Potassium thiocyanate, Sodium Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	4	277	PEG 400, Potassium thiocyanate, Sodium Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K