1X6J
Crystal structure of ygfY from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-09-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54179 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 40.400, 42.700, 84.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
Rwork | 0.188 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.600 |
Data scaling software | SCALEPACK |
Phasing software | BEAST |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.328 |
Number of reflections | 5225 | |
<I/σ(I)> | 7.8 | 2 |
Completeness [%] | 97.1 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.1 M sodium Malonate, 0.1 M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |