1X54
Crystal structure of asparaginyl-tRNA synthetase from Pyrococcus horikoshii complexed with asparaginyl-adenylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.071, 68.221, 75.706 |
Unit cell angles | 90.00, 117.18, 90.00 |
Refinement procedure
Resolution | 30.290 - 1.450 |
R-factor | 0.16903 |
Rwork | 0.168 |
R-free | 0.19157 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b8a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.296 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 97438 | |
Completeness [%] | 96.1 | 78.1 |
Redundancy | 4.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |