1X3Z

Structure of a peptide:N-glycanase-Rad23 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	93
Detector technology	CCD
Collection date	2005-02-21
Detector	KODAK
Wavelength(s)	1.0721
Spacegroup name	P 31 2 1
Unit cell lengths	128.801, 128.801, 128.376
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.23737
Rwork	0.236
R-free	0.27010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.644
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	30868
Completeness [%]	97.4	91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	sodium chloride, MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K