1X3Z
Structure of a peptide:N-glycanase-Rad23 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-02-21 |
| Detector | KODAK |
| Wavelength(s) | 1.0721 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 128.801, 128.801, 128.376 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.23737 |
| Rwork | 0.236 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 30868 | |
| Completeness [%] | 97.4 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | sodium chloride, MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
