1X3K
Crystal structure of a hemoglobin component (TA-V) from Tokunagayusurika akamusi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-14 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.144, 74.855, 33.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.140 - 1.640 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX (2000.1) |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.027 | 0.068 |
| Number of reflections | 20177 | |
| Completeness [%] | 97.3 | 94.2 |
| Redundancy | 6.98 | 6.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 3350, Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
