1X2W
Crystal Structure of Apo-Habu IX-bp at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-11 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.121, 37.831, 55.828 |
| Unit cell angles | 90.00, 110.46, 90.00 |
Refinement procedure
| Resolution | 34.680 - 2.290 |
| Rwork | 0.214 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.086 | 0.272 |
| Number of reflections | 11631 | |
| <I/σ(I)> | 7.3 | 2.3 |
| Completeness [%] | 95.7 | 89.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | AMMONIUM SULPHATE, RUBIDIUM ACETATE, PEGMME 2000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
