1X2I

Crystal Structure Of Archaeal Xpf/Mus81 Homolog, Hef From Pyrococcus Furiosus, Helix-hairpin-helix Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-21
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	39.942, 56.889, 68.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.450
R-factor	0.2234
Rwork	0.222
R-free	0.23830
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	27454
Completeness [%]	96.9	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	trypsin, Na-Malonate, pH 7.0, EVAPORATION, temperature 293K
1	EVAPORATION	7	293	trypsin, Na-Malonate, pH 7.0, EVAPORATION, temperature 293K