1X25
Crystal Structure of a Member of YjgF Family from Sulfolobus Tokodaii (ST0811)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-01 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 54.980, 54.980, 221.557 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.030 - 2.000 |
| R-factor | 0.14787 |
| Rwork | 0.146 |
| R-free | 0.18799 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qd9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.488 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.051 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.151 |
| Number of reflections | 16882 | |
| <I/σ(I)> | 9.8 | 5.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | PEG 10000, Bis-Tris, ammonium acetate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
