1X1V
Structure Of Banana Lectin- Methyl-Alpha-Mannose Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.820, 80.820, 148.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.920 - 2.450 |
| R-factor | 0.2257 |
| Rwork | 0.224 |
| R-free | 0.26034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.070 | 0.533 |
| Number of reflections | 21617 | |
| Completeness [%] | 99.0 | 99.3 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | zinc acetate dihydrate, sodium cacodylate, 1,6-hexanediol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
