1X1S
Crystal structure of M-Ras in complex with GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 109.711, 109.711, 69.426 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.000 - 2.200 |
| R-factor | 0.22332 |
| Rwork | 0.222 |
| R-free | 0.25585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.171 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 0.307 |
| Number of reflections | 13000 | |
| <I/σ(I)> | 7.6 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.8 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG8000, sodium cacodylate, magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
