1X1I
Crystal Structure of Xanthan Lyase (N194A) Complexed with a Product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Detector technology | CCD |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.802, 90.677, 77.920 |
Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
Resolution | 47.360 - 1.800 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 68423 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PEG4000, Ammonium Formate, Sodium Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |