1X1E
Crystal Structure of TT0495 protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-23 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 62.712, 62.712, 111.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.450 - 1.760 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vl8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.065 | |
| Number of reflections | 22749 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 295 | Sodium acetate, sodium formate, pH 4.6, microbatch, temperature 295K |
