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1X10

Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192A) from a Hyperthermophile, Pyrococcus furiosus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL40B2
Synchrotron siteSPring-8
BeamlineBL40B2
Temperature [K]100
Detector technologyCCD
Collection date2000-12-15
DetectorADSC QUANTUM 4
Wavelength(s)1.0
Spacegroup nameP 21 21 21
Unit cell lengths47.440, 103.961, 187.494
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.000
Rwork0.227
R-free0.24810
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ioi
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.030
High resolution limit [Å]2.0002.000
Rmerge0.0480.083
Number of reflections62100
<I/σ(I)>3315.2
Completeness [%]96.486
Redundancy5.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.6298PEG4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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