1X0V
Crystal Structure of Homo Sapien Glycerol-3-Phosphate Dehydrogenase 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9798, 0.9800, 0.900 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 113.488, 113.488, 155.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 88158 | |
| Completeness [%] | 98.8 | 85.3 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1M tri-Sodium Citrate dihydrate, 0.5M Ammonium Sulfate, 1.0M Lithium sulfate monohydorate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
