1X0I
Crystal Structure of the Acid Blue Form of Bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.640, 102.640, 109.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.910 - 2.300 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.520 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.500 |
| Number of reflections | 15527 | |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 2 | 277 | ammonium sulfate, pH 2.00, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
