1X0G
Crystal Structure of IscA with the [2Fe-2S] cluster
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-07 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.900 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 77.472, 102.788, 137.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.20302 |
Rwork | 0.201 |
R-free | 0.24150 |
Structure solution method | MAD |
RMSD bond length | 0.028 |
RMSD bond angle | 2.503 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 19261 | |
<I/σ(I)> | 39.4 | 3.5 |
Completeness [%] | 96.0 | 96.6 |
Redundancy | 12.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 2.5M NaCl, 0.1M Potassium phosphate pH6.2, 5MM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 2.5M NaCl, 0.1M Potassium phosphate pH6.2, 5MM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |