1X04
Crystal structure of endophilin BAR domain (mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45PX |
Synchrotron site | SPring-8 |
Beamline | BL45PX |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2004-11-27 |
Detector | RIGAKU JUPITER |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 129.077, 129.077, 97.963 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.24 |
Rwork | 0.238 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.074 | 0.350 |
Number of reflections | 17780 | |
<I/σ(I)> | 20.7 | 5.5 |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 8 | 277 | NaCl, ethylene glycol, glycerol, benzamizine/HCl, Tris, DTT, pH 8.0, MICRODIALYSIS, temperature 277K |