1X03

Crystal structure of endophilin BAR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	90
Detector technology	CCD
Collection date	2004-07-04
Detector	RIGAKU JUPITER
Wavelength(s)	0.9797
Spacegroup name	I 41
Unit cell lengths	127.424, 127.424, 99.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.238
Rwork	0.236
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.030
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.066	0.363
Number of reflections	14464
<I/σ(I)>	22.9	4.5
Completeness [%]	99.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	277	NaCl, ethylene glycol, glycerol, benzamizine/HCl, Hepes, DTT, pH 7.4, MICRODIALYSIS, temperature 277K
1	MICRODIALYSIS	7.4	277	NaCl, ethylene glycol, glycerol, benzamizine/HCl, Hepes, DTT, pH 7.4, MICRODIALYSIS, temperature 277K
1	MICRODIALYSIS	7.4	277	NaCl, ethylene glycol, glycerol, benzamizine/HCl, Hepes, DTT, pH 7.4, MICRODIALYSIS, temperature 277K