1WZB

Crystal structure of the collagen triple helix model [{HYP(R)-HYP(R)-GLY}10]3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1 21 1
Unit cell lengths	18.405, 19.113, 90.961
Unit cell angles	90.00, 94.26, 90.00

Refinement procedure

Resolution	9.990 - 1.500
R-factor	0.17152
Rwork	0.170
R-free	0.20051
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k6f
Data reduction software	CLYSTAL (CLEAR VER1.35)
Data scaling software	CrystalClear (V. 1.35 (MSC/RIGAKU))
Phasing software	ACORN
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.240	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.057	0.088
Number of reflections	10316
<I/σ(I)>	27.3	15.8
Completeness [%]	98.2	98.6
Redundancy	5.65	5.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	277	HHG from 23.0 mg/ml peptide solution, 20% PEG 4000, 20% isopropanol, 0.2M Tris HCL buffer, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K