1WZ7
Crystal structure of enhancer of rudimentary homologue (ERH)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97904, 0.97939, 0.96000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.249, 43.260, 92.061 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 24.730 - 2.100 |
| Rwork | 0.219 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 19595 | |
| <I/σ(I)> | 9.5 | 4.6 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M Bis-Tris, 22% PEG 3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
