1WZ2
The crystal structure of Leucyl-tRNA synthetase and tRNA(leucine) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.546, 231.127, 118.181 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 3.210 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% isopropanol, 200mM tri-sodium citrate, 100mM sodium cacodylate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | isopropanol | ||
| 2 | 1 | 1 | tri-sodium citrate | ||
| 3 | 1 | 1 | sodium cacodylate buffer | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | tri-sodium citrate | ||
| 6 | 1 | 2 | sodium cacodylate buffer | ||
| 7 | 1 | 2 | HOH |
