1WZ1
Crystal structure of the Fv fragment complexed with dansyl-lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2004-01-01 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.630, 81.630, 74.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.600 - 1.850 |
Rwork | 0.216 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dlf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.187 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.600 | 1.820 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.078 | 0.421 |
Number of reflections | 23960 | |
<I/σ(I)> | 28.4 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 8000, magnecium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |