1WYX
The Crystal Structure of the p130Cas SH3 Domain at 1.1 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.071, 56.193, 37.924 |
Unit cell angles | 90.00, 116.22, 90.00 |
Refinement procedure
Resolution | 34.100 - 1.140 |
R-factor | 0.17651 |
Rwork | 0.176 |
R-free | 0.19109 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.308 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.100 | 1.150 |
High resolution limit [Å] | 1.100 | 1.100 |
Completeness [%] | 93.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | acetate, magnesium chloride, PEG 8000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |