1WYW
Crystal Structure of SUMO1-conjugated thymine DNA glycosylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.212, 70.420, 106.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.087 | 0.350 * |
Number of reflections | 19228 | |
<I/σ(I)> | 8 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19 | 19.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 * | 20 * | 25% PEG3350, 0.1M Tris, 0.2M Magnesium Chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | HEPES | 25 (mM) | |
2 | 1 | 1 | 150 (mM) | ||
3 | 1 | 1 | dithiothreitol | 1 (mM) | |
4 | 1 | 1 | protein | 20 (mg/ml) | |
5 | 1 | 2 | PEG3350 | 25 (%) | |
6 | 1 | 2 | 0.2 (M) | ||
7 | 1 | 2 | Tris-HCl | 0.1 (M) |