1WYI
human triosephosphate isomerase of new crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-10 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.564, 70.883, 141.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.200 - 2.200 |
R-factor | 0.266 |
Rwork | 0.247 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNX (2002) |
Refinement software | CNX (2002) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.200 |
High resolution limit [Å] | 2.200 |
Number of reflections | 23600 |
Completeness [%] | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | gel-tube under microgavity | 8 | 293 | PEG4000, MgCl2, pH 8.0, gel-tube under microgavity, temperature 293K |