1WYI

human triosephosphate isomerase of new crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-12-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.564, 70.883, 141.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.200 - 2.200
R-factor	0.266
Rwork	0.247
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNX (2002)
Refinement software	CNX (2002)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.200
High resolution limit [Å]	2.200
Number of reflections	23600
Completeness [%]	96.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	gel-tube under microgavity	8	293	PEG4000, MgCl2, pH 8.0, gel-tube under microgavity, temperature 293K