1WY4

Chicken villin subdomain HP-35, K65(NLE), N68H, pH5.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2004-07-09
Detector	RIGAKU RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	19.720, 40.225, 75.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.177
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yrf
RMSD bond length	0.020
RMSD bond angle	0.020
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	4299
<I/σ(I)>	38.7	14.2
Completeness [%]	93.5	58.5
Redundancy	26.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.1	293	1ul 50mg/ml peptide plus 1ul (200mM AmCitrate, 20% PEF3350), pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.10