1WY1
Crystal Structure of the PH0671 protein from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-03 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.188, 78.722, 52.013 |
Unit cell angles | 90.00, 108.60, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nog |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.055 | 0.450 |
Number of reflections | 45212 | |
<I/σ(I)> | 11.9 | 1.82 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.5 | 4.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.7 | 295 | PEG3350, magnesium acetate, pH 7.7, microbatch, temperature 295K |