1WXW

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	93
Detector technology	CCD
Collection date	2004-09-27
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.979081, 0.979378, 0.9640
Spacegroup name	C 1 2 1
Unit cell lengths	252.704, 45.962, 139.640
Unit cell angles	90.00, 100.08, 90.00

Refinement procedure

Resolution	45.830 - 2.550
R-factor	0.223
Rwork	0.223
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	96483
Completeness [%]	95.7	74.7
Redundancy	1.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	35% PPG P400, 0.1M NaAcetate, 3% 1.6 Hexanediol, 10mM cysteine, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K