1WXQ
Crystal Structure of GTP binding protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-06 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 0.9791, 0.97942, 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.962, 142.999, 78.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.2659 |
| Rwork | 0.266 |
| R-free | 0.29165 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.811 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.076 | |
| Number of reflections | 11523 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.1 | 98.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 295 | PEG 4000, HEPES, Dioxane, pH 7.5, microbatch, temperature 295K |
