1WXC
Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.6199 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.664, 96.734, 54.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.200 |
R-factor | 0.1799 |
Rwork | 0.180 |
R-free | 0.20830 |
Structure solution method | MIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 0.028 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.260 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.085 | 0.366 |
Number of reflections | 102888 | |
<I/σ(I)> | 16.4 | 4.6 |
Completeness [%] | 97.8 | 99 |
Redundancy | 8.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | peg3350, sodium nitrate, hepes, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |