1WX5
Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein in the monoclinic crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-09-05 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.850, 93.200, 65.590 |
Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.020 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wxc |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.680 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.125 | 0.278 |
Number of reflections | 46161 | |
<I/σ(I)> | 7.3 | 3.4 |
Completeness [%] | 99.6 | 98.9 |
Redundancy | 5.01 | 4.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG4000, ammonium chloride, hepes, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |