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NGF IN COMPLEX WITH DOMAIN 5 OF THE TRKA RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	160
Spacegroup name	P 1 21 1
Unit cell lengths	58.245, 53.735, 77.101
Unit cell angles	90.00, 107.34, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.189 *
Rwork	0.189
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bet
RMSD bond length	0.010
RMSD bond angle	1.910
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.071 *	0.355 *
Number of reflections	23361
<I/σ(I)>	7.7
Completeness [%]	99.8	98.7
Redundancy	4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		0.1 (M)
3	1	drop	bicine	0.1 (M)
4	1	reservoir	PEG3350	24 (%)
5	1	reservoir	citrate	0.1 (M)