1WWW
NGF IN COMPLEX WITH DOMAIN 5 OF THE TRKA RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 160 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.245, 53.735, 77.101 |
| Unit cell angles | 90.00, 107.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bet |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.910 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 * | 0.355 * |
| Number of reflections | 23361 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | bicine | 0.1 (M) | |
| 4 | 1 | reservoir | PEG3350 | 24 (%) | |
| 5 | 1 | reservoir | citrate | 0.1 (M) |
