1WWL
Crystal structure of CD14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Detector technology | CCD |
Collection date | 2004-02-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.519, 117.705, 102.395 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.790 - 2.500 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.27800 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 29282 | |
Completeness [%] | 94.9 | 78.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100mM NaHepes, 1.9M Li2SO4, 5mM NiCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |