1WWK
Crystal structure of phosphoglycerate dehydrogenase from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-13 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 222.417, 46.163, 66.258 |
Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
Resolution | 37.480 - 1.900 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.24600 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.442 |
Number of reflections | 53427 | |
<I/σ(I)> | 11.8 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.7 | 295 | PEG4000, NAD, pH 5.7, microbatch, temperature 295K |
1 | MICROBATCH | 5.7 | 295 | PEG4000, NAD, pH 5.7, microbatch, temperature 295K |
1 | MICROBATCH | 5.7 | 295 | PEG4000, NAD, pH 5.7, microbatch, temperature 295K |