1WWB
LIGAND BINDING DOMAIN OF HUMAN TRKB RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Collection date | 1996-03-01 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 68.340, 68.340, 124.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.247 * |
| Rwork | 0.247 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wwc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.019 |
| Number of reflections | 115960 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | 4 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.3 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | bicine | 17 (mM) | |
| 4 | 1 | drop | AEBSF | 0.83 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 5.8 (%sat) | |
| 6 | 1 | drop | MPD | 0.17 (%(v/v)) | |
| 7 | 1 | reservoir | ammonium sulfate | 45 (%sat) | |
| 8 | 1 | reservoir | MPD | 1 (%(v/v)) |
