1WW8
Crystal Structure of malic enzyme from Pyrococcus horikoshii Ot3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-23 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 0.979059, 0.979418, 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.457, 85.681, 172.786 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.079 | |
Number of reflections | 29793 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.1 | 295 | PEG 20K, Tris-HCl, pH 8.1, microbatch, temperature 295K |