1WW6
Agrocybe cylindracea galectin complexed with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-09-21 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.506, 57.344, 64.471 |
| Unit cell angles | 90.16, 111.62, 89.88 |
Refinement procedure
| Resolution | 9.990 - 2.200 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 2.280 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 30302 | |
| <I/σ(I)> | 42.89 | 10.73 |
| Completeness [%] | 91.0 | 83.7 |
| Redundancy | 1.94 | 0.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammomium sulfate, lactose, pipes, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
