1WVU
Crystal structure of chitinase C from Streptomyces griseus HUT6037
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 3 |
Unit cell lengths | 127.850, 127.850, 127.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.470 - 2.450 |
R-factor | 0.17494 |
Rwork | 0.172 |
R-free | 0.22272 |
Structure solution method | MIRAS |
RMSD bond length | 0.020 |
RMSD bond angle | 1.646 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.640 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.041 | 0.125 |
Number of reflections | 24823 | |
<I/σ(I)> | 14 | 5.2 |
Completeness [%] | 96.2 | 75.5 |
Redundancy | 4.5 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Sodium chloride, Sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |