1WVT
Crystal structure of uncharacterized protein ST2180 from Sulfolobus tokodaii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 117.577, 117.577, 79.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
Rwork | 0.221 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1woz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.294 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 25266 | |
<I/σ(I)> | 10.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.7 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | Ammonium sulfate, PEG400, HEPES buffer, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |