1WVN
Crystal Structure of domain 3 of human alpha polyC binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 33.400, 71.000, 29.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.510 - 2.100 |
R-factor | 0.214 |
Rwork | 0.190 |
R-free | 0.24758 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ec6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.279 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.510 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.042 | 0.258 |
Number of reflections | 3817 | |
<I/σ(I)> | 27.4 | 3.5 |
Completeness [%] | 98.9 | 87.7 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | HEPES, lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |