1WVN

Crystal Structure of domain 3 of human alpha polyC binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-03-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	33.400, 71.000, 29.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.510 - 2.100
R-factor	0.214
Rwork	0.190
R-free	0.24758
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ec6
RMSD bond length	0.012
RMSD bond angle	1.279
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.510	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.042	0.258
Number of reflections	3817
<I/σ(I)>	27.4	3.5
Completeness [%]	98.9	87.7
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	HEPES, lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K