1WVD
HIV-1 Dis(Mal) Duplex CoCl2-Soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.168, 59.168, 63.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.930 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.060 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Number of reflections | 5330 | |
| Completeness [%] | 99.1 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, spermine, KCl, MgCl2, Na Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | Na Cacodylate | ||
| 2 | 1 | 1 | spermine | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | Na Cacodylate | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | MgCl2 |
