1WV9
Crystal Structure of Rhodanese Homolog TT1651 from an Extremely Thermophilic Bacterium Thermus thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-08 |
Detector | RIGAKU |
Wavelength(s) | 0.96200, 0.97906, 0.97938 |
Spacegroup name | P 31 |
Unit cell lengths | 45.218, 45.218, 79.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.900 - 2.000 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.190 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | oil microbatch | 6.3 | 293 | PEG4000, MES, Sodium chloride, Tris-HCl, pH 6.3, oil microbatch, temperature 293K |