1WV4
X-ray Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in tetragonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-27 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 54.420, 54.420, 164.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.380 - 2.600 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dnl |
RMSD bond length | 0.022 |
RMSD bond angle | 2.100 |
Data reduction software | bioteX |
Data scaling software | bioteX |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.380 | 2.800 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.063 | 0.249 |
Number of reflections | 14262 | |
<I/σ(I)> | 12.4 | 2.7 |
Completeness [%] | 90.8 | 91.3 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Na Hepes, ammonium phosphate, potassium phosphate, mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |