1WUD
E. coli RecQ HRDC domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97941 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.304, 64.611, 98.114 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.227 |
Rwork | 0.225 |
R-free | 0.26344 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.780 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 12636 | |
Completeness [%] | 99.6 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 4000, MES, TCEP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |