1WU8
Crystal structure of project PH0463 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-29 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 0.979056 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 138.756, 138.756, 139.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.660 - 2.600 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.109 | 0.421 |
| Number of reflections | 42753 | |
| <I/σ(I)> | 8.5 | 3.6 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 13.3 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbach | 5.9 | 291 | 44 v/v (%) MPD, 0.1M Citr., 10M Dioxane, pH 5.9, Microbach, temperature 291K |
