1WU7
Crystal structure of histidyl-tRNA synthetase from Thermoplasma acidophilum
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97899, 0.97948, 0.96112 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.696, 101.734, 167.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.26300 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 41644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | sodium/potassium phosphate, PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |