1WTY
Crystal structure of a probable nucleotidyl transferase protein from thermus thermophilus HB8
Replaces: 1WJ8Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-27 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.90000, 0.97899, 0.979333 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.320, 116.250, 123.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.370 - 2.200 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.28000 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.240 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.064 | 0.361 |
Number of reflections | 153969 | |
<I/σ(I)> | 12.3 | 3.9 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 4.64 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG6000, 0.05M Imidazole-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |