1WTO
Hyperthermophile chromosomal protein SAC7D double mutant V26F/M29F in complex with DNA GCGATCGC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.11634 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.900, 47.170, 60.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.500 |
| R-factor | 0.2145 |
| Rwork | 0.214 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.351 |
| Number of reflections | 16734 | |
| <I/σ(I)> | 22.7 | 4.17 |
| Completeness [%] | 95.7 | 94.2 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 400, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 400, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | Tris buffer | ||
| 3 | 1 | 2 | PEG 400 | ||
| 4 | 1 | 2 | Tris buffer |
