1WTE
Crystal structure of type II restrcition endonuclease, EcoO109I complexed with cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.103, 71.879, 203.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.640 - 1.900 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.22324 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.794 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.770 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 55952 | |
| Completeness [%] | 93.5 | 89.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | PEG 20000, sodium acetate, 6-aminocaproic acid, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | 6-aminocaproic acid | ||
| 3 | 1 | 1 | PEG 20000 | ||
| 4 | 1 | 2 | sodium acetate | ||
| 5 | 1 | 2 | PEG 20000 |
